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采用Ｃ（１８）小柱净化尿样，富集尿中被测物丙烯酰胺代谢物──巯基尿酸（乙酰半胱氨酸－Ｓ－丙酰胺）。处理过的尿样用Ｅｌｌｍａｎ＇ｓ反应分光光度法测定。用本方法做游离巯基测定的批内变异系数为０．１４％～１．６％（ｎ＝６），总巯基测定的批内变异系数为１．０％～５．２％（ｎ＝６），方法的加标回收率为８２．７％～８４．４％。检出下限：游离巯基尿酸为０．００８ｍｍｏｌ／Ｌ，总巯基尿酸为０．０１３ｍｍｏｌ／Ｌ。本方法测定了９例非接触者尿样，巯基尿酸浓度范围０．０５６～０．０８６ｍｍｏｌ／Ｌ，４１例接触丙烯酰胺工人的尿，巯基尿酸浓度范围０．０６１～０．３７４ｍｍｏｌ／Ｌ。
C (18) cartridge was used to purify the urine sample and enrich the metabolite of acrylamide, namely thiol uric acid (acetylcysteine-S-propionamide) in the urine. Treated urine samples were measured by Ellman’s reaction spectrophotometry. The intra-assay coefficient of variation (ICC) of this method was 0.14% -1.6% (n = 6) and the intra-assay coefficient of variation of total sulfhydryl was 1.0% -5.2% (n = 6 ), The recovery rate of the method was 82.7% ~ 84.4%. Lower limit of detection: Free thiol uric acid 0.008mmol / L, total thiourea uric acid 0.013mmol / L. The urine samples of 9 non-contact persons were measured by this method. The concentrations of thiourea and uric acid were 0.056-0.086 mmol / L, while the urine samples of 41 patients exposed to acrylamide were 0.061-0.374 mmol / L.