实验观察表明,四种脱氧核苷三磷酸与靛玉红预保温未见抑制作用;酶与该药预保温后抑制率比对照组增加近一倍;而酶和DNA一起与之预保温后抑制率则增加两倍。提示靛玉红可能通过形成酶-模板-药物三元复合物而发挥最大抑制作用的。 Experimental observations showed that there was no inhibitory effect on the pre-incubation of four deoxynucleoside triphosphates and indirubin; the inhibition rate after pre-incubation of the enzyme and the drug nearly doubled compared with the control group; and the enzyme and DNA were inhibited after pre-incubation with indirubin. The rate is doubled. It is suggested that indirubin may exert its maximum inhibitory effect through the formation of enzyme-template-drug ternary complexes.