目的:研究漆树叶对动物的皮肤刺激性与致敏性。方法:采集新鲜漆树叶(不含叶轴)冻存后粉碎,室温下将漆树叶屑用蒸馏水制成糊剂,叶和水的比例为1:1。皮肤刺激实验:新西兰纯种家兔6只,脊柱两侧备皮(3cm×3cm)后,将漆树叶糊1.0g涂敷在左侧皮肤上,覆盖4h后清洗,右侧皮肤涂0.5ml蒸馏水作为对照。清洗后1、24、48、72h观察涂敷漆树叶糊部位皮肤反应并评分。皮肤致敏实验:白化豚鼠50只,分为实验组(20只,雌雄各半)、阳性对照组(20只,雌雄各半)和阴性对照组(10只,雌雄各半)。脊柱两侧备皮(2cm×2cm)。实验开始即刻和第7、14天时,实验组背部左侧备皮处涂敷0.8g漆树叶糊,阳性对照组涂敷2.5%2,4-二硝基氯苯0.2g,阴性对照组涂蒸馏水0.4ml,均覆盖6h后清洗;实验第28天时,实验组和阴性对照组豚鼠背部左侧备皮处涂敷0.8g漆树叶糊,阳性对照组涂敷1.0%2,4-二硝基氯苯0.2g,覆盖6h后清洗。其后24、48h分别计算致敏率、判定致敏程度。结果:皮肤刺激实验:有3只家兔在涂敷漆树叶糊后皮肤出现红斑,评分平均值为0.67分(24h),刺激反应程度为轻度。皮肤致敏实验:实验组与阴性对照组所有豚鼠涂敷漆树叶糊后24、48h均未出现红斑、水肿等反应,致敏反应积分平均值为0,致敏率为0,致敏程度为弱。阳性对照组豚鼠出现红斑、水肿,48h皮肤致敏反应总积分平均值为3.65分,致敏率为100%,致敏程度为极强。结论:漆树叶对实验动物皮肤有轻度刺激性,无致敏性。 Objective: To study the skin irritation and allergenicity of paint leaves on animals. Methods: Fresh lacquer leaves (without leaf shaft) were harvested and crushed. The leaves of lacquer were made into paste by distilled water at room temperature. The ratio of leaf to water was 1: 1. Skin irritation test: 6 New Zealand purebred rabbits, after skin preparation (3 cm × 3 cm) on both sides of the spine, 1.0 g of the leaf gum paste was applied on the left skin. After covering for 4 hours and washing, the right skin was coated with 0.5 ml of distilled water as comparison. After cleaning 1,24,48,72 h observe the application area of ​​the paint leaf laccase reaction and score. Skin sensitization test: 50 guinea pigs were divided into experimental group (20 males and females), positive control group (20 males and females) and negative control group (10 males and females). Spine on both sides of the skin (2cm × 2cm). At the beginning of the experiment and on the 7th and 14th days, 0.8g of leaf gum was coated on the left side of the back of the experimental group and 0.2g of 2,4-dinitrochlorobenzene in the positive control group. The negative control group was coated with distilled water 0.4ml, were covered after 6h cleaning; experimental day 28, the experimental group and the negative control group guinea pigs on the left side of the preparation skin coated with 0.8g leaf gum, the positive control group coated with 1.0% 2,4-dinitro chloride Benzene 0.2g, after 6h cover cleaning. After 24, 48h were calculated sensitization rate, to determine the degree of sensitization. Result: Skin irritation test: Three rabbits showed erythema on the skin after the application of La leaf gum, the average score was 0.67 (24h), and the degree of irritation was mild. Skin sensitization test: In experimental group and negative control group, all guinea pigs did not show erythema, edema and other reactions at 24 and 48h after application of leaf gum pasteurized. The sensitivities were 0 and the sensitization rate was 0 weak. In the positive control group, erythema and edema occurred in guinea pigs, and the average score of skin allergic reaction at 48h was 3.65, the sensitization rate was 100% and the sensitization degree was extremely strong. CONCLUSION: Lacquered leaves are slightly irritating to the skin of experimental animals without allergenicity.