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[Objective]To identify genuine medicinal materials Lycium barbarum L.by ISSR-PCR molecular marker technique on the level of DNA molecular.[Method]Suitable primers were screened out from 50 ISSR primers(Inter-simple sequence repeat),and then used in PCR amplification and electrophoretic analysis for distinguishing Lycium barbarum L.from No.1 and male sterility by finding specific sites.[Result]1 ISSR primer could amplify polymorphic bands that suitable for distinguishing Lycium barbarum L.from No.1 and male sterility.[Conclusion]ISSR-PCR molecular marker technique was quick and reliable,which could be used in identification of Lycium barbarum L..
[Objective] To identify genuine medicinal materials Lycium barbarum L. by ISSR-PCR molecular marker technique on the level of DNA molecular. [Method] Suitable primers were screened out from 50 ISSR primers (Inter-simple sequence repeat), and then used in PCR amplification and electrophoretic analysis for distinguishing Lycium barbarum L. from No.1 and male sterility by finding specific sites. [Result] 1 ISSR primer could amplify polymorphic bands that suitable for distinguishing Lycium barbarum L. from No.1 and male sterility. [ Conclusion] ISSR-PCR molecular marker technique was quick and reliable, which could be used in identification of Lycium barbarum L ..