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目的：建立丹参细胞培养物中二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡＡ等丹参酮的ＨＰＬＣ测定法。方法：采用ＹＷＧＧ１８Ｈ３７柱，以甲醇水（８０∶２０）为流动相，在２５４ｎｍ处检测。结果：测定了不同丹参细胞系中４种丹参酮的含量。结论：在不同丹参细胞中，液体培养丹Ⅰ８细胞系中各丹参酮的含量较高。
OBJECTIVE: To establish an HPLC method for the determination of tanshinones such as dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ and tanshinone ⅡA in Salvia miltiorrhiza cell culture. Methods: YWG G18H37 column with methanol water (80:20) as the mobile phase at 254nm at the detection. Results: Four tanshinones were determined in different Salvia miltiorrhiza cell lines. Conclusion: In different Salvia miltiorrhiza cells, the content of each tanshinone in liquid culture Dan Ⅰ8 cell line is higher.