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目的探讨B7共刺激分子在人类恶性血液病细胞系中的表达以及激活T淋巴细胞的免疫反应。方法用流式细胞术检测HL-60、NB4、Jurkat、U937、Raji、Daudi、K562等细胞HLA抗原和B7分子表达,同时检测经Ara-C刺激前后B7分子的表达,用RT-PCR方法检测经Ara-C刺激前后激活T淋巴细胞分泌细胞因子IFN-γ mRNA。结果HL-60、NB4、Jurkat、U937、Raji、Daudi、K562等恶性血液病细胞株均表达或高表达HLA抗原和B7-2分子,低表达或不表达B7-1分子,经Ara-C刺激后细胞株B7分子的表达明显增强,并能有效地激活T淋巴细胞表达高水平的IFN-γ mRNA。结论大多数人类恶性血液病细胞株均不表达或低表达B7分子,但是B7-1可能在肿瘤免疫中起重要作用。
Objective To investigate the expression of B7 costimulatory molecules in human malignant hematological malignancies and the immune response of activated T lymphocytes. Methods The expressions of HLA antigens and B7 molecules in HL-60, NB4, Jurkat, U937, Raji, Daudi and K562 cells were detected by flow cytometry. The expression of B7 protein was detected by RT-PCR The cytokine IFN-γ mRNA was secreted by activated T lymphocytes before and after Ara-C stimulation. Results The HL-60, NB4, Jurkat, U937, Raji, Daudi, K562 and other hematological malignant cell lines expressed or highly expressed HLA antigens and B7-2 molecules, with or without B7-1 expression. The expression of the B7 molecule in the post-cell strain was significantly enhanced, and the high level of IFN-γ mRNA was efficiently activated in T lymphocytes. Conclusion Most human hematological malignant hematological malignancies do not express or down-regulate B7 molecules, but B7-1 may play an important role in tumor immunity.