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目的:探讨中药颤复宁血清对6羟基多巴(6OHDA)诱导的大鼠嗜铬细胞瘤细胞(PC12)凋亡的抑制作用。方法:用血清药理学方法制备中药血清,加到经6OHDA处理的PC12细胞培养液中,用四甲基偶氮唑蓝(MTT)法检测细胞存活率,并用免疫组织化学染色方法和图像分析检测酪氨酸羟化酶(TH)含量及半胱氨酸蛋白酶3(Caspase3)阳性凋亡细胞数。结果:颤复宁中药血清能有效地提高PC12细胞的存活率,对6OHDA诱导的凋亡有明显抑制作用。与6OHDA损伤组比较,颤复宁血清组TH阳性细胞平均吸光度增高(P<0.01),Caspase3阳性细胞数明显降低(P<0.01)。结论:中药颤复宁血清对PC12细胞具有直接的神经营养作用,能提高细胞存活率,且对6OHDA诱导的细胞凋亡具有抑制作用。
Objective: To investigate the inhibitory effect of Shenfuning serum on the apoptosis of rat pheochromocytoma (PC12) cells induced by 6-OHDA. Methods: The serum of traditional Chinese medicine was prepared by serum pharmacology and added to the culture medium of PC12 cells treated with 6OHDA. The viability of the cells was detected by MTT assay and detected by immunohistochemical staining and image analysis. Tyrosine hydroxylase (TH) content and caspase 3 positive apoptotic cell number. RESULTS: The serum concentration of Chanfuning could effectively increase the survival rate of PC12 cells and inhibit the apoptosis induced by 6OHDA. Compared with the 6OHDA group, the average absorbance of TH positive cells was increased (P<0.01) and the number of Caspase3 positive cells was significantly decreased (P<0.01). Conclusion: Chinese medicine Chanfuning serum has direct neurotrophic effect on PC12 cells, can increase the cell survival rate, and has an inhibitory effect on 6OHDA-induced apoptosis.