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无鞭毛期利什曼原虫仅寄生于脊椎动物的单核吞噬细胞内。作者试图用体外培养的人成纤维细胞作为原虫的宿主,以研究两者的关系。墨西哥利什曼原虫亚马逊亚种取自感染的金色仓鼠,取下其非溃疡性的足垫皮组织,在洛克氏液中捣碎,离心,弃去组织碎片,收集原虫。再以洛克氏液1,000g离心洗涤10分钟,共3次。最后混悬于HamF10液内备用。从正常人获得成纤维细胞株,保存于含15%灭活小牛血清的Ham F10中,并置于含5%CO_2,37℃的孵箱内培养。在培养瓶或盖玻片上培养单层成纤维细胞,24小时后,按10∶1的比例加入利什曼原
Non-flagellated Leishmania parasites only in vertebrate mononuclear phagocytes. The authors attempted to study the relationship between the two using human fibroblasts cultured in vitro as hosts for protozoa. The Leishmania mexicana subsp. Amazoni was taken from an infected golden hamster and its non-ulcerous footpad tissue was removed, mashed in Rock’s solution, centrifuged, tissue debris was removed and the protozoa collected. Centrifugal washing with Lockard’s solution 1,000g for 10 minutes for 3 times. Finally, suspended in HamF10 liquid spare. Fibroblast strains were obtained from normal individuals and stored in Ham F10 with 15% inactivated calf serum and incubated in an incubator containing 5% CO 2 at 37 ° C. Monolayer fibroblasts were cultured on culture flasks or coverslips and 24 hours later, leishmanine was added at a ratio of 10: 1