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利用水稻基因芯片筛选到1个在水稻孕穗期和抽穗期穗中的相对表达量明显高于苗期、孕穗期和抽穗期叶片中相对表达量的基因Os MTG3,该基因在孕穗期穗中受低温、干旱诱导上调表达,而在抽穗期穗中受低温诱导下调表达。把该基因ATG密码子上游1.8kb左右的DNA片段预测为启动子区,并将其命名为p Os MTG3。用PCR技术克隆该启动子,并以GUS基因作为报告基因,在水稻中分析了该启动子在不同时期不同组织中的表达特性。GUS组织化学染色分析表明:p Os MTG3启动子能启动GUS基因在水稻根、芽、茎、穗中表达,而不能启动GUS基因在叶片中表达,是一个全新的叶片特异性不表达的启动子。
A rice gene chip was screened to a relative expression level in rice at the booting and heading stages, which was significantly higher than that at the seedling, booting and heading stages. Os MTG3 was expressed in the booting stage Low temperature, drought induced up-regulated expression, while in the panicle induced by low temperature down-regulated expression. A DNA fragment of about 1.8 kb upstream of the ATG codon of the gene was predicted as a promoter region and named as p Os MTG3. The promoter was cloned by PCR and the expression of this promoter was analyzed in different tissues of rice at different times using GUS as a reporter gene. GUS histochemical staining showed that pUS MTG3 promoter can activate GUS gene expression in rice roots, buds, stems and spikes, but can not start GUS gene expression in leaves, is a new leaf-specific promoter is not expressed .