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目的 探讨 2型糖尿病患者外周血单个核细胞中转录因子 κB(NuclearfactorkappaB ,NF κB)的活性与肾功能损害的关系。方法 应用凝胶电泳迁移率实验 (EMSA)测定了 41例 2型糖尿病患者 (A组 )和 1 0例健康正常人 (D组 )外周血单个核细胞中NF κB的活性 ,并根据有无肾脏病变将糖尿病患者分为糖尿病无肾病组 (B组 )和糖尿病肾病组 (C组 )。结果 与D组比较 ,A、B、C三组NF κB活性显著增强 (P均 <0 0 5) ,其中C组活性最高 ,B、C两组间NF κB活性也有差别 (P <0 0 5)。结论 2型糖尿病患者外周血单个核细胞中NF κB活性显著增强 ,尤其是伴有肾脏病变的患者 ,NF κB与糖尿病肾病的发生、发展有关。
Objective To investigate the relationship between the activity of nuclear factor kappa B (NF κB) and renal dysfunction in peripheral blood mononuclear cells of type 2 diabetic patients. Methods The activity of NF-κB in peripheral blood mononuclear cells of 41 type 2 diabetic patients (group A) and 10 healthy controls (group D) was measured by electrophoretic mobility shift assay (EMSA) Diabetic patients were divided into diabetic nephropathy group (group B) and diabetic nephropathy group (group C). Results Compared with group D, the NF κB activities of group A, B and C were significantly increased (P <0.05), of which group C was the highest, and there was a difference in NF κB activity between group B and C (P <0.05) ). Conclusion The NF κB activity in peripheral blood mononuclear cells of patients with type 2 diabetes significantly increased, especially in patients with nephropathy, NF κB and diabetic nephropathy, the occurrence and development.