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目的探讨雌激素受体G蛋白耦联受体30(GPR30)在大鼠下颌下腺组织中的表达,为进一步研究此受体对下颌下腺的功能调节提供理论依据。方法取SD大鼠4只,腹腔麻醉后切取下颌下腺,采用免疫组织化学和原位杂交方法进行定位研究;从下颌下腺组织中分别提取总RNA,应用RT-PCR方法获得GPR30基因的cDNA核心序列,并进行序列分析。结果大鼠下颌下腺浆液性腺泡及颗粒曲管上皮细胞呈GPR30免疫反应阳性,阳性物质分布于细胞质和细胞膜上,细胞核呈阴性反应。上述细胞同样含有GPR30mRNA杂交信号,信号物质亦分布于细胞质内,细胞核呈阴性反应。经序列分析发现,从大鼠下颌下腺组织中扩增出GPR30基因的特异性条带。结论大鼠下颌下腺浆液性腺泡上皮细胞能够表达雌激素受体GPR30,说明其可能是雌激素快速作用的靶器官。
Objective To investigate the expression of estrogen receptor G-protein coupled receptor 30 (GPR30) in the rat submandibular gland tissue, and to provide a theoretical basis for further study on the functional regulation of this receptor on the submandibular gland. Methods Four SD rats were obtained. The submandibular glands were excised after intraperitoneal anesthesia. Immunohistochemistry and in situ hybridization were used for localization study. The total RNA was extracted from the submandibular gland tissue, and the cDNA core sequence of GPR30 gene was obtained by RT-PCR , And sequence analysis. Results The rat submandibular gland serous acinar and granulocytic epithelial cells showed positive GPR30 immunoreactivity. The positive substances distributed on the cytoplasm and the cell membrane, and the nucleus was negative. The above cells also contain the GPR30 mRNA hybridization signal. The signal substances are also distributed in the cytoplasm, and the nucleus is negative. Sequence analysis revealed that a specific band of GPR30 gene was amplified from rat submandibular gland tissue. Conclusion The rat submandibular gland serous acinar epithelial cells can express the estrogen receptor GPR30, indicating that it may be the target organ of rapid estrogen.