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目的:利用四氧二苯二氧杂环己二烯(TCDD,二噁英)和苯并(a)芘(B[a]P)致大鼠肺癌的动物模型,研究肺癌变过程中CYPIA1基因mRNA和蛋白水平的表达。方法:应用RT-PCR方法及荧光免疫法检测大鼠正常肺组织、癌前病变组织和肺癌组织中CYPIA1基因mRNA的表达及CYPIA1基因在肺组织蛋白水平的表达,评价CYPIA1基因在肺癌变过程的作用。结果:CYPIA1 mRNA/β-actin比值在TCDD、TCDD+B[a]P、B[a]P致癌组及对照组分别为0.956 4±0.328 2、1.153 3±0.432 6、0.252 4±0.103 2和0.093 6±0.032 7;在大鼠正常肺组织、正常增生、癌前病变和肺癌组织中分别为0.105±0.098、0.235±0.108、1.127±0.295和1.564±0.421。结果表明,CYPIA1mRNA在TCDD+B[a]P、TCDD致癌组有较强表达,在肺癌前病变和肺癌组织中有较强表达;CYP1A1蛋白表达灰度值在TCDD、TC-DD+B[a]P、B[a]P致癌组及对照组分别为41.76±4.82、46.28±4.32、31.02±3.16和18.75±2.27,在大鼠肺正常组织、正常增生、癌前病变和肺癌组织中分别为19.77±2.74、26.71±3.32、43.95±3.98和47.38±4.96。结果表明,CYP1A1蛋白在TCDD+B[a]P和TCDD致癌组有较强表达,在肺癌前病变和肺癌组织中有较强表达。结论:TCDD和B[a]P对CYPIA1有较好的诱导作用,CYPIA1高表达与肺癌的发生有着密切的关系,其在肺癌前病变和肺癌组织中的高表达,可能是肺癌前病变、肺癌发生的一个标志。
OBJECTIVE: To investigate the effect of CYPIA1 gene in the process of lung carcinogenesis in animal models of lung cancer in rats induced by tetrazoliumdioxide (TCDD, dioxin) and benzo (a) pyrene (B [a] P) mRNA and protein expression. Methods: The mRNA expression of CYPIA1 in normal lung tissue, precancerous lesions and lung cancer tissues was detected by RT-PCR and fluorescence immunoassay, and the expression of CYPIA1 in lung tissue protein was assessed by RT-PCR and fluorescence immunoassay. effect. Results: The ratios of CYPIA1 mRNA / β-actin in TCDD, TCDD + B [a] P, B [a] P carcinomas and controls were 0.956 4 ± 0.328 2,1.153 3 ± 0.432 6,0.252 4 ± 0.103 2 and 0.093 6 ± 0.032 7, respectively, which were 0.105 ± 0.098, 0.235 ± 0.108, 1.127 ± 0.295 and 1.564 ± 0.421 respectively in normal lung tissues, normal proliferation, precancerous lesions and lung cancer tissues of rats. The results showed that CYPIA1mRNA was strongly expressed in TCDD + B [a] P, TCDD carcinogenesis group, and was strongly expressed in precancerous lesions of lung and lung cancer tissues. The expression of CYP1A1 protein in TCDD, TC-DD + B [a ] P, B [a] P carcinogenicity group and the control group were 41.76 ± 4.82,46.28 ± 4.32,31.02 ± 3.16 and 18.75 ± 2.27, respectively, in normal lung tissue, normal proliferation, precancerous lesions and lung cancer tissues were 19.77 ± 2.74, 26.71 ± 3.32, 43.95 ± 3.98 and 47.38 ± 4.96. The results showed that CYP1A1 protein was strongly expressed in TCDD + B [a] P and TCDD carcinogenesis group, and was strongly expressed in precancerous lesions and lung cancer tissues. CONCLUSION: TCDD and B [a] P have a good effect on CYPIA1. CYPIA1 overexpression is closely related to the occurrence of lung cancer. The high expression of CYPIA1 in lung cancer and lung cancer may be a precancerous lesion of lung and lung cancer A sign of what happened.