论文部分内容阅读
目的观察中国汉族T2DM患者中葡萄糖依赖性促胰岛素肽(GIP)及其受体(葡萄糖依赖性促胰岛素肽受体,GIPR)基因多态性与冠心病风险的相关性。方法纳入666例T2DM患者,其中冠心病组390例,对照组276例。根据HapMapCHB数据库选取单体型标记的单核苷酸多态性(tagSNPs)。GIPR基因共4个tag-SNPs,分别为rs1800437、rs11671664、rs4803846和rs12709891。应用聚合酶链式反应-限制性内切酶片断长度多态性方法,对GIPR基因tag-SNPs进行等位基因和基因型频率分析,研究GIPR基因多态性与冠心病风险的关系。结果两组GIPR基因SNPs等位基因频率比较,差异无统计学意义。冠心病组和对照组rs1800437、rs11671664、rs4803846和rs12709891等位基因频率分别为79.8%vs 82.5%,62.6%vs 59.3%,89.0%vs 89.3%和48.8%vs 50.4%。两组GIPR基因rs1800437和rs4803846单体型组合比较,差异无统计学意义。冠心病组和对照组单体型组合GG、GC和AG频率分别为69.2%vs 72.0%,19.8%vs 17.3%和10.6%vs 10.5%。结论中国T2DM患者中,未发现GIPR基因多态性与冠心病发生风险相关的SNPs,提示GIP受体基因变异可能不是中国T2DM患者冠心病风险的主要决定因素。
Objective To investigate the association between glucose-dependent insulinotropic peptide (GIP) and its receptor (glucose-dependent insulinotropic peptide receptor, GIPR) gene polymorphism and risk of coronary heart disease in Chinese Han population with T2DM. Methods A total of 666 T2DM patients were enrolled. Among them, 390 cases were coronary heart disease group and 276 cases in control group. Single haplotype tag SNPs were selected based on the HapMapCHB database. There are 4 tag-SNPs in GIPR gene, which are rs1800437, rs11671664, rs4803846 and rs12709891, respectively. The alleles and genotype frequencies of tag-SNPs in GIPR gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The relationship between GIPR gene polymorphism and the risk of coronary heart disease was investigated. Results There was no significant difference in allele frequency between two groups of GIPR gene SNPs. The frequencies of rs1800437, rs11671664, rs4803846 and rs12709891 in CHD group and control group were 79.8% vs 82.5%, 62.6% vs 59.3%, 89.0% vs 89.3% and 48.8% vs 50.4%, respectively. Two groups of GIPR gene rs1800437 and rs4803846 haplotype combination, the difference was not statistically significant. The haplotype GG, GC and AG frequencies of CHD group and control group were 69.2% vs 72.0%, 19.8% vs 17.3% and 10.6% vs 10.5%, respectively. Conclusion No SNPs associated with GIPR gene polymorphism and coronary heart disease risk were found in T2DM patients, suggesting that GIP receptor gene mutation may not be the major determinant of coronary heart disease risk in T2DM patients.