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目的:探讨养心通脉有效成分部位方(YTⅡ)促血管生成的作用靶点及其机理。方法:制备养心通脉有效部位方(YTⅡ)和各对照组(YTⅠ组、SBW组、bFGF组、KX组)含药血清,并进行心肌缺血模型大鼠心脏血管内皮细胞的培养;观察比较在血管生成的过程中YTⅡ及各对照组对心脏血管内皮细胞“增殖”“、迁移”和“管腔结构形成”不同环节的干预作用。结果:各组药物干预后,心肌缺血大鼠冠状血管内皮细胞的增殖率、迁移率、成管率3类指标均呈现出bFGF组>YTⅡ组>YTⅠ组>SBW组>NS组>KX组递减的趋势。YTⅡ和bFGF对血管生成的不同环节均有明显的促进作用,且显著优于其他各组。结论:养心通脉有效部位方(YTⅡ)益气活血,能刺激血管内皮细胞“增殖”“、迁移”和“管腔结构形成”不同环节,促进缺血肌的血管生成。
OBJECTIVE: To explore the target and mechanism of angiogenesis induced by YTII. Methods: The drug-containing serum of YXII and control groups (YTI group, SBW group, bFGF group, and KX group) were prepared, and the culture of cardiac vascular endothelial cells was performed in rats with myocardial ischemia. To compare the effects of YTII and various control groups on the vascular endothelial cell “proliferation”, “migration” and “luminal structure formation” in the process of angiogenesis. RESULTS: After intervention of each group of drugs, the proliferation rate, migration rate, and tube formation rate of coronary vascular endothelial cells in the three groups of rats exhibited bFGF group> YTII group> YTI group> SBW group> NS group> KX group. Decreasing trend. YTII and bFGF have obvious promoting effects on different aspects of angiogenesis, and are significantly better than other groups. Conclusion: YTII can improve blood circulation, stimulate proliferation of vascular endothelial cells, migration, and formation of lumen structure, and promote the blood vessels of ischemic muscles. generate.