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一、各种细胞器的分离方法:差速离心法例1在低温条件下,将叶片置于研钵中,加入某种溶液研磨后,将细胞碎片和细胞器用离心法进行分离,第一次分离成沉淀P_1(含细胞核和细胞壁碎片)和上层液S_1;随后又将S_1分离成沉淀P_2(含叶绿体)和上层液S_2;第三次离心将S_2分离成沉淀P_3(含线粒体)和上层液S_3;最后一次将S_3分离成沉淀P_4(含核糖体)和上层液体S_4。(1)含DNA最多的部分是。
First, the separation of a variety of organelles: differential centrifugation Law 1 under low temperature conditions, the leaves placed in a mortar, after adding a solution of grinding, the cell debris and organelles were separated by centrifugation, the first separation into P_1 (including cell nucleus and cell wall fragments) and supernatant S_1 were precipitated; S_1 was then separated into P_2 (including chloroplast) and supernatant S_2; the third centrifugation separated S_2 into P_3 (containing mitochondria) and supernatant S_3; The last separation of S_3 into precipitated P_4 (including ribosomes) and the upper liquid S_4. (1) The part containing the most DNA is.