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本刊1983第5期发表的“紫外分光光度法测定盐酸普鲁卡因丁卡因注射液的含量”一文中提到A_(291)=0.0671C可用于盐酸普鲁卡因注射液的含量测定。我们在实际应用中发现,用该方法(下称直接法)不能准确反映盐酸普鲁卡因的实际含量。其原因是盐酸普鲁卡因注射液不稳定,受pH与温度的影响。文献报道[温玉鳞:药学通报9(9):363,1963],该注射液在20～70℃时,温度每升高10℃,水解速度增加3.1倍,因此,该注射液在制备和贮存过程中,普鲁卡因易水解为对氨基苯甲酸,而对氨基苯甲酸在波长290±1nm处也有吸收(附图)。因此,不能采用直接法测定盐酸普鲁卡因的含量。美国药典20版采用加碱-氯仿提取后,再用紫外分光光度法测定盐酸普鲁卡因的含量。我们将上述方法与直接法进行了比较,发现用直接法测定的含量较
The fifth issue 1983 published “UV spectrophotometric determination of procaine hydrochloride tetracaine injection” in a paper mentioned A_ (291) = 0.0671C can be used for procaine hydrochloride injection Determination of content. We found in practice, using this method (hereinafter referred to as direct method) can not accurately reflect the actual content of procaine hydrochloride. The reason is that procaine hydrochloride injection is unstable, affected by pH and temperature. Reported in the literature [Wen Yu scales: Bulletin 9 (9): 363,1963], the injection at 20 ~ 70 ℃, the temperature increased by 10 ℃, the hydrolysis rate increased 3.1 times, therefore, the injection in the preparation and Procaine is easily hydrolyzed to para-aminobenzoic acid during storage, whereas para-aminobenzoic acid is also absorbed at a wavelength of 290 ± 1 nm (with photos). Therefore, the direct method can not be used for the determination of procaine hydrochloride. The United States Pharmacopoeia 20 version with alkali - chloroform extraction, and then UV spectrophotometric determination of procaine hydrochloride content. We compared the above method with the direct method and found that the direct method was more effective