论文部分内容阅读
目的研究枸杞多糖(LBP)对氟化钠(NaF)致小鼠成釉细胞DNA损伤的影响。方法 离体培养小鼠成釉细胞,2个处理因素为NaF染毒(含浓度为0.00、0.25、0.50、1.00、2.00、4.00 mmol/L 6个水平)和LBP预处理(含质量浓度为0.00、100.00、200.00、400.00 mg/L 4个水平),6×4析因设计,2因素各水平全面组合共分24组,以浓度为0.00mmol/L NaF+质量浓度为0.00 mg/L LBP组为对照组。小鼠成釉细胞经LBP预处理24 h后,再给予NaF染毒,24 h后收获细胞,采用单细胞凝胶电泳检测DNA损伤情况。结果 分别以0.50、1.00、2.00、4.00 mmol/L NaF单独染毒的小鼠成釉细胞尾长、Olive尾距、尾部DNA%、尾长/头长值均高于对照组(P<0.05,P<0.01或P<0.001)。析因设计方差分析结果显示,LBP和NaF存在交互作用,差异均有统计学意义(P<0.001)。经质量浓度分别为100.00、200.00、400.00mg/L的LBP预处理后,在不同NaF染毒剂量的条件下,多数尾长、Olive尾距、尾部DNA%、尾长/头长值等DNA损伤指标值均低于相应剂量NaF单独染毒时的指标值(P<0.05,P<0.01或P<0.001)。结论 一定剂量NaF可致小鼠成釉细胞DNA损伤,LBP对氟诱导的小鼠成釉细胞DNA损伤起到一定的保护作用。
Objective To investigate the effects of LBP on DNA damage in ameloblasts induced by sodium fluoride (NaF) in mice. Methods The mouse ameloblasts were cultured in vitro. The two treatment factors were NaF (containing 6 concentrations of 0.00,0.25,0.50,1.00,2.00,4.00 mmol / L) and LBP pretreatment (with concentration of 0.00 , 100.00, 200.00, 400.00 mg / L, 4 levels), 6 × 4 factorial design. The two factors were totally divided into 24 groups at a concentration of 0.00mmol / L NaF + concentration of 0.00 mg / Control group. The ameloblasts of mice were pretreated with LBP for 24 hours and then treated with NaF. After 24 hours, the cells were harvested, and the DNA damage was detected by single cell gel electrophoresis. Results The tail length, tail length, tail DNA length and tail length / head length of ameloblasts were significantly higher in the control group (0.50, 1.00, 2.00, 4.00 mmol / L NaF) P <0.01 or P <0.001). Factorial analysis of variance analysis showed that there was interaction between LBP and NaF, the differences were statistically significant (P <0.001). After pretreatment with LBP of 100.00, 200.00 and 400.00 mg / L, respectively, DNA damage of most tail length, Olive tail length, tail DNA%, tail length / head length under different doses of NaF The index values were lower than those of the corresponding doses of NaF alone (P <0.05, P <0.01 or P <0.001). Conclusion A certain dose of NaF can induce DNA damage of ameloblasts in mice, and LBP can protect DNA from ameloblasts induced by fluoride in mice.