论文部分内容阅读
目的探讨一氧化氮(No)及一氧化氮合酶(NOS)在肝硬化患者血清中的变化。方法硝酸盐还原酶法。因为NO在血清中以稳定的离子硝酸盐离子(NO_3-)和亚硝酸盐离子(NO_2-)存在,因此硝酸盐离子和亚硝酸盐离子可以准确地反映NO合成量。同时,根据NOS可以催化L-精氨酸生成NO,而NO与二价铁结合成有色物的性质,对NOS进行检测。结果我们对30例肝硬化患者及30例正常人血清中NO及NOS进行测定,其结果显示如下:肝硬化如血清中NO:(50.4±19.0)μmol/L,NOS:(6.72±2.37)u/ml,正常组血清中NO:(34.5±16.8)μmol/L,NOS:(3.89±1.35)μ/ml,经过T检验结果为P<0.01。其结果显示,正常人血清中的NO及NOS均低于肝硬化组,而NO与NOS的含量变化基本成正相关。因为肝脏本身存在NOS,可以合成并释放NO。结论肝硬化患者血清中NO及NOS的含量均明显高于正常对照组,提示可能与肝硬化的代谢、肝功能的损伤程度等有密切关系。
Objective To investigate the changes of serum nitric oxide (NO) and nitric oxide synthase (NOS) in cirrhotic patients. Methods Nitrate reductase method. Because NO is present in the serum as stable ion nitrate (NO 3 -) and nitrite (NO 2 -), nitrate and nitrite ions accurately reflect NO production. At the same time, NOS can catalyze the formation of NO by L-arginine, and the combination of NO and bivalent iron into the color of the material, the detection of NOS. Results The serum levels of NO and NOS were measured in 30 patients with cirrhosis and 30 normal controls. The results are as follows: (1) the serum levels of NO: (50.4 ± 19.0) μmol / L, NOS: (6.72 ± 2.37) u / ml, NO in normal group (34.5 ± 16.8) μmol / L and NOS: (3.89 ± 1.35) μ / ml, respectively. The result of T test was P <0.01. The results showed that the normal serum NO and NOS were lower than the cirrhosis group, while the NO and the content of NOS basically positive correlation. Because of the presence of NOS in the liver itself, NO can be synthesized and released. Conclusions The serum levels of NO and NOS in patients with cirrhosis are significantly higher than those in normal controls, suggesting that they may be closely related to the metabolism of liver cirrhosis and the degree of liver damage.