流行性出血热病毒的细胞培养

来源 :兰州大学学报 | 被引量 : 0次 | 上传用户：mbranger

【摘要】

：

用灭活的流行性出血热病毒(EHFV)L99株感染传代培养的Vero-E6细胞,第4 d 50%的Vero-E6细胞胞浆内可查到对EHFV鼠血清的特异性荧光,第7～10 d阳性细胞数达到 100%.用感染第3 d的

【作者】

：

王美亮彭静王超美高珊周学良

【机构】

：

解放军473医院,兰州生物制品研究所,,甘肃省人民医院,

【出处】

：

兰州大学学报

【发表日期】

：

2004年期

【关键词】

：

流行性出血热病毒 Vero -E6细胞细胞培养

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用灭活的流行性出血热病毒(EHFV)L99株感染传代培养的Vero-E6细胞,第4 d 50%的Vero-E6细胞胞浆内可查到对EHFV鼠血清的特异性荧光,第7～10 d阳性细胞数达到 100%.用感染第3 d的细胞培养上清大批量盲种,第7～10 d荧光强度均达到++++.电镜下病毒呈圆形或卵圆形,外膜为电子致密层,界面清楚,包绕着比较疏松的内浆,内浆中有若干个颗粒丝状结构.

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流行性出血热病毒的细胞培养

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