目的探讨邻苯二甲酸二(2-乙基)己酯(DEHP)短期重复暴露对雄性大鼠生殖毒性及睾丸脂质过氧化水平的影响。方法将初断乳清洁级Wistar雄性大鼠40只按体重随机分为DEHP低、中、高剂量组[DEHP的染毒剂量分别为10、100、1 000 mg/(kg.d)]和溶剂对照组(玉米油),每组10只。采用灌胃方式进行染毒,连续染毒30 d。测定睾丸组织中丙二醛(MDA)和还原型谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,蛋白定量采用酚试剂法。结果染毒第4周,DEHP高剂量染毒组体重、睾丸质量及其脏器系数低于溶剂对照组,差异均有统计学意义(P<0.05或P<0.01)。DEHP中、高剂量染毒组睾丸组织中MDA含量高于溶剂对照组,差异均有统计学意义(P<0.05),且MDA含量随着DEHP染毒剂量的增加而升高。DEHP高剂量染毒组睾丸组织中GSH含量、SOD和GSH-Px活力低于溶剂对照组,差异有统计学意义(P<0.05),且SOD和GSH-Px活力均随着DEHP染毒剂量的增加而降低。结论DEHP短期重复暴露对初断乳大鼠的生殖系统发育具有明显的毒性作用。DEHP及其代谢产物可使机体产生氧自由基,导致脂质过氧化水平升高,是DEHP致青春前期及青春期雄性大鼠生殖毒性的可能机制之一。 Objective To investigate the effects of short-term repeated exposure of di-2-ethyl hexyl phthalate (DEHP) on reproductive toxicity and testicular lipid peroxidation in male rats. Methods 40 Wistar male rats were randomly divided into DEHP low, middle and high dose groups [DEHP exposure dose was 10, 100, 1 000 mg / (kg · d)] and solvent Control group (corn oil), 10 rats in each group. Gavage by way of exposure, continuous exposure 30 d. The contents of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in testis tissue were measured. Phenol reagent method. Results At the 4th week of exposure, body weight, testicular weight and organ coefficient of DEHP high dose exposure group were lower than those of solvent control group (P <0.05 or P <0.01). The content of MDA in testis of DEHP group was higher than that in solvent control group (P <0.05), and MDA content increased with the increase of DEHP dose. The activities of GSH, SOD and GSH-Px in testis tissue of high-dose DEHP exposure group were lower than that of solvent control group (P <0.05), and the activities of SOD and GSH-Px were all increased with DEHP dose Increase and decrease. Conclusion Short-term repeated DEHP exposure has significant toxic effects on reproductive system development in weanling rats. DEHP and its metabolites can make the body produce oxygen free radicals, leading to elevated levels of lipid peroxidation, is one of the possible mechanisms of DEHP induced reproductive toxicity in pre-and adolescent male rats.