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利用Ｄｏｔｂｌｏｔ和流式细胞术，研究了分化诱导剂维甲酸，ＤＭＳＯ对ＨＬ－６０细胞及其抗性亚型抗药性程度的影响，表明１μｍｏｌ·ｍｌ~(－１)ＲＡ作用ＨＬ－６０及其亚型２４ｈ，ＭＤＲ１ｍＲＮＡ明显增高，但流式检测多药抗性细胞系对Ｒｈｏ－１２３的外排有所下降。２％ＤＭＳＯ作用ＨＬ－６０及其亚型２４ｈ，流式细胞术检测显示各细胞系对Ｒｈｏ－１２３的外排明显增强，提示ＲＡ虽然提高ＭＤＲ１基因的表达，但可能通过磷酸化／脱磷酸化方式抑制Ｐｇｐ－１７０功能的表达，而ＤＭＳＯ能诱导完整功能的Ｐｇｐ表达。
The effects of differentiation inducer retinoic acid and DMSO on the drug resistance of HL-60 cells and their resistant subtypes were studied by Dotblot and flow cytometry, indicating that the effect of 1 μmol · ml -1 RA on HL-60 and its Subtype 24h, MDR1mRNA was significantly increased, but flow assay multidrug resistance cell line Rho-123 efflux decreased. The effect of 2% DMSO on HL-60 and its subtypes 24h and flow cytometry showed that the efflux of Rho-123 in each cell line was significantly enhanced, suggesting that although RA enhances the expression of MDR1 gene, it may be through phosphorylation / dephosphorylation The manner inhibited the expression of Pgp-170 function, whereas DMSO induced the intact function of Pgp expression.