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Objective:To compare the transferring efficiency and killing effects of one time and continuous mediation with GE7,a non-viral targeted delivery system,in transfection of thymidine kinase gene of herpes simplex virus(HSV-tk)into ovarian cancer cells.Methods:GE7 was used to prepare recombinants with β-galactosidase(β-gal)and HSV1-tk;the recombinants were then used to transfect human ovarian cancer line CaOV3 once and continuously.β-gal staining was used to compare the efficiencies of one time and continuous mediation with GE7 system.Ganciclovior(GCV)was introduced into HSV1-tk transfected ovarian cells.Through drawing the cell growth curve and flow cytometry,the killing effects of GCV on once and continuously GE7/HSV1-tk transfected cells were observed.Results:We found that the one time and continuous exogenous gene transfer efficiencies were about 80% and 85%,respectively When 1 μg/mL GCV was used to treat ovarian cell transfected with HSV1-tk gene,growth inhibiting rates of ovarian cells of one time and continuous transferring were 82% and 90%,respectively;their apoptosis indices were 15 and 30,respectively.Under same GCV concentration.continuous mediation of GE7/pCMV-tk transfection into ovarian cancer cells had more significant inhibitory effect than one time mediation(P＜0.05).Conclusion:Compared with one time mediation,continuous mediation of transfection with GE7 gene delivery system has higher efficiency.Continuous mediation of GE7/HSV1-tk/GCV therapeutic gene system has more powerful killing effect.