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以聚环氧乙烷-聚环氧丙烷-聚环氧乙烷三嵌段共聚物(P123)与离子型助表面活性剂{十六烷基三甲基溴化铵(CTAB)、十二烷基硫酸钠(SDS)或N-肉豆蔻酰-D-丙氨酸(C14-D-A)}为结构导向剂,合成了蠕虫状孔道的介孔SiO2,并在P123/C14-D-A/TMAPS(N-三甲氧基硅丙基-N,N,N-三甲基氯化铵)合成反应体系中,研究了扩孔剂聚乙二醇(PEG)-400的不同添加量对蠕虫状孔道的扩孔效果.合成的产物分别用X-射线衍射(XRD)、扫描电镜(SEM)、透射电镜(TEM)和N2吸附进行表征.此外,利用溶剂萃取法对扩孔后的蠕虫状介孔SiO2进行处理,得到不同孔径的季铵盐官能团化介孔SiO2,分别用于漆酶的固定化;并对固定化酶的稳定性、所需的最佳pH值以及降解2,4-DCP的重复利用性进行了研究.通过比较研究发现,介孔SiO2孔径与漆酶分子直径的匹配性是影响固定化漆酶比活及降解2,4-DCP效率的重要因素.
Polyethylene oxide - polypropylene oxide - polyethylene oxide triblock copolymer (P123) and ionic co-surfactant {cetyl trimethyl ammonium bromide (CTAB), dodecane Mesoporous SiO2 in worm-like pore was synthesized and characterized on the surface of P123 / C14-DA / TMAPS (N - trimethoxysilylpropyl-N, N, N-trimethylammonium chloride) synthesis reaction system, the study of the expanding agent polyethylene glycol (PEG) -400 different amount of wormhole expansion Pore effect.The synthesized products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and N2 adsorption, respectively.In addition, the mesoporous silica , Respectively, to obtain quaternary ammonium functionalized mesoporous SiO2 with different pore diameters, respectively, for immobilization of laccase; and the stability of the immobilized enzyme, the optimal pH value required, and the repeated utilization of degraded 2,4-DCP Through the comparative study, it was found that the match between the pore size of mesoporous silica and the diameter of laccase is an important factor affecting the specific activity of immobilized laccase and the efficiency of degrading 2,4-DCP.