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使用根据转基因Bt176玉米中的外源基因CryIA(b)和内源基因Zein设计的引物和TaqMan荧光探针和ABIPRISM 770 0定量PCR仪对玉米粉中Bt176玉米的含量进行了定量检测 ,建立了Bt176玉米参照样品CryIA(b)和Zein的Ct值之差与样品中Bt176玉米含量之间的标准曲线和线性回归方程 ,并对进口的未知样品进行了检测
The content of Bt176 maize in corn meal was quantitatively determined by using the primers designed according to the exogenous gene CryIA (b) and the endogenous gene Zein in the transgenic Bt176 maize and the TaqMan fluorescent probe and the ABIPRISM 770 0 quantitative PCR machine. Bt176 The standard curve and linear regression equation between the difference between the Ct values of the corn reference samples CryIA (b) and Zein and the Bt176 corn content in the samples were analyzed, and unknown imported samples were also tested