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淋巴细胞在体外培养中,在植物血凝素(Phytohaemagglutinin,PHA)的作用下,激活并转化为“母细胞”。PHA激活的是T淋巴细胞,B细胞则不受其影响。T淋巴细胞在机体的细胞免疫过程中有重要作用。近年来,用PHA体外激活淋巴细胞,引起淋巴细胞转化,应用于肿瘤的予后与治疗,在国内外日益受到重视。淋巴细胞体外激活的效应,即淋巴细胞的转化率,可用两种方法检测:一种是以形态变化作指标,另一种是3~H——TdR的方法来检测。测定的体外培养后第3天或第5天的淋巴细胞转化率在不同研究者虽有差异,大约为55—90％,平均近70％。国内目前
In vitro culture, lymphocytes are activated by Phytohaemagglutinin (PHA) and transformed into “blast cells.” PHA is activated T lymphocytes, B cells are not affected. T lymphocytes in the body’s cellular immune process has an important role. In recent years, the use of PHA in vitro activation of lymphocytes, causing lymphocyte transformation, applied to the tumor after treatment with more and more attention at home and abroad. In vitro activation of lymphocytes, lymphocyte transformation rate, can be detected by two methods: one is based on morphological changes as indicators, and the other is 3 ~ H - TdR method to detect. The measured lymphocyte transformation rate on day 3 or 5 after in vitro culture varied from 55% to 90% in different investigators, with an average of nearly 70%. Domestic at present