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目的:阐明炎症肠道疾病(inlammatory bowel disease,IBD)中肺表面活化蛋白A(pulmonary surfactant protein A,SP-A)相似分子的分布特征和CD68阳性巨噬细胞的免疫反应。方法:外科手术获得的结肠组织标本来自布朗大学医学院罗得岛医院病理科,应用免疫组织化学方法检测IBD患者肠道组织中SP-A相似分子的表达。结果:SP-A相似分子分布在肠道上皮、肠道绒毛表面,连接组织血管和某些炎症细胞。具有SP-A相似分子和CD68阳性表达的巨噬细胞数量炎症区域明显增加,高于正常组织。而免疫荧光双标记实验显示,一些CD68阳性巨噬细胞可以表达SP-A相似分子免疫反应。结论:SP-A是肺脏一种重要的宿主防御因子。
Objective: To elucidate the distribution of pulmonary surfactant protein A (SP-A) -like molecules and the immune response of CD68 positive macrophages in inflammatory bowel disease (IBD). Methods: Colorectal tissues were obtained from the Rhode Island Hospital of Brown University Medical School. The expression of SP-A similar molecules in intestinal tract of IBD patients was detected by immunohistochemistry. Results: SP-A similar molecules distributed in the intestinal epithelium, intestinal villi surface, connecting tissue and some inflammatory cells. The number of macrophages with SP-A similar molecules and positive expression of CD68 was significantly increased, higher than that of normal tissues. Immunofluorescence double labeling experiments showed that some CD68 positive macrophages could express SP-A similar molecular immune responses. Conclusion: SP-A is an important host defense factor of lung.