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背景与目的:肿瘤原位移植模型已成为肿瘤实验动物学的主要载体,但目前尚缺乏方便可靠的方法监测模型体内肿瘤的生长变化。本文评价β-人绒毛膜促性腺激素(βhumanchorionicgonadotropin,β-HCG)系统对肿瘤原位模型内肿瘤生长的监测作用。方法:将pclone-β-HCG质粒稳定转染的人卵巢癌细胞系A2780-CG制成裸小鼠原位移植模型,通过连续检测鼠尿中β-HCG/肌酐值,绘制曲线,间接了解小鼠体内肿瘤生长规律,以及顺铂腹腔化疗后,该比值对顺铂抗瘤效果的监测作用。结果:β-HCG系统转染瘤细胞A2780-CG的成瘤时间和母代相同。小鼠尿中β-HCG/肌肝值与体内瘤重量呈正相关(r=0.98);顺铂腹腔化疗后,β-HCG/肌酐值呈进行性下降。结论:β-HCG系统可无创、敏感地监测卵巢癌原位移植模型中肿瘤生长规律,为该模型在肿瘤治疗中的应用提供简便的监测手段。
BACKGROUND & OBJECTIVE: Tumor orthotopic transplantation has become the main carrier of tumor zoology. However, there is no convenient and reliable method to monitor the growth of tumor in vivo. This article evaluates the role of β-human chorionic gonadotropin (β-HCG) in monitoring tumor growth in situ in tumor models. Methods: The human ovarian cancer cell line A2780-CG stably transfected with pclone-β-HCG plasmid was made into a orthotopic implantation model in nude mice. The curve of β-HCG / creatinine in rat urine was drawn and curve Tumor growth in mice, and cisplatin after intraperitoneal chemotherapy, the ratio of cisplatin on the anti-tumor effect of the monitoring effect. Results: The tumorigenic time of A2780-CG transfected with β-HCG system was the same as that of the mother generation. The urinary β-HCG / muscle mass had a positive correlation with the tumor weight in vivo (r = 0.98). The β-HCG / creatinine level decreased progressively after intraperitoneal cisplatin chemotherapy. CONCLUSION: The β-HCG system can monitor the tumor growth in non-invasive and sensitive ovarian carcinoma orthotopic implantation model and provide a simple and convenient monitoring method for the application of β-HCG in tumor therapy.