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目的 研究阿克他利 (Acta)的抗炎和免疫调节作用。方法 观察佐剂性关节炎 (AA)大鼠给药后原发和继发性足肿胀、免疫功能及前列腺素释放水平的变化 ,并检测Acta体外对正常小鼠T细胞亚群的影响。结果 Acta (10 ,30与90mg·kg-1)灌胃给药 (ig)对佐剂性关节炎 (AA)大鼠的原发炎症反应无明显影响 ,但可明显抑制AA大鼠继发性的关节肿胀 ,改善AA大鼠多发性关节炎病变症状。体外研究发现 ,Acta (10 ,30与 90mg·kg-1)ig能使AA大鼠低下的ConA诱导的增殖反应和IL 2恢复接近正常 ,对AA大鼠过高的IL 1产生有明显的抑制作用 ,但对大鼠PMΦ产生的PGE2 水平无明显影响。体外培养发现 ,Acta (1～ 10 0 μmol·L-1)可抑制正常小鼠ConA诱导的Th细胞 ,促进ConA诱导的Ts细胞。结论 Acta无明显的抗炎作用 ,但具有免疫调节作用 ,通过调节细胞免疫功能 ,实现对AA大鼠的治疗作用。
Objective To investigate the anti-inflammatory and immunomodulatory effects of Acta. Methods The changes of primary and secondary paw edema, immune function and prostaglandin release in adjuvant arthritis (AA) rats were observed. The effects of Acta on T lymphocyte subsets in normal mice were observed. Results Administration of Acta (10, 30 and 90 mg · kg-1) had no effect on the primary inflammatory response in adjuvant arthritis (AA) rats by intragastric administration but inhibited the secondary Swollen joints and improve the symptoms of multiple arthritis in AA rats. In vitro study, Acta (10, 30 and 90 mg · kg-1) ig can make AA rat low ConA-induced proliferative response and IL 2 recovery close to normal, AA rat over-high IL 1 production was significantly inhibited But had no significant effect on the level of PGE2 produced by PMΦ in rats. In vitro, Acta (1 ~ 100 μmol·L -1) inhibited ConA-induced Th cells in normal mice and promoted ConA-induced Ts cells. Conclusion Acta has no obvious anti-inflammatory effect, but it has immunomodulatory effects and can achieve therapeutic effects on AA rats by regulating cellular immune function.