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目的研究婴儿人巨细胞病毒(HCMV)感染的不同临床表现与巨细胞病毒糖蛋白 B(gB)基因型的关系。方法 HCMV 感染且有临床症状的婴儿107例,无临床症状的婴儿25例,用套式 PCR 法扩增 gB 基因片段,限制性片段长度多态性分析(RFLP)对 gB 基因进行分型,并对部分标本通过测序进行验证。结果在有临床症状的107例婴儿中,检出 gB Ⅰ型53例,gBⅡ型20例,gBⅢ型18例,gB Ⅰ、Ⅱ混合型7例,gB Ⅰ、Ⅲ混合型5例,gBⅡ、Ⅲ混合型4例,未发现 gB Ⅳ型。其中有肝功能损害的53例婴儿中检出 gB Ⅰ型36例,gBⅡ型5例,gB Ⅲ型7例,gB Ⅰ、Ⅱ混合型3例,gBⅠ、Ⅲ混合型2例,gB Ⅰ型明显占多数(P<0.05)。在无临床症状的25例婴儿中检出 gB Ⅰ型10例,gBⅡ型6例,gBⅢ型8例,gB Ⅰ、Ⅱ混合型1例。对132例婴儿中的24例标本进行测序,结果与相应的典型菌株序列比较,相似性为97.06%～99.64%。结论 RFLP 对 HCMV 的 gB 基因分型明确可靠。有肝功能损害的 HCMV 感染婴儿,gB Ⅰ型为优势基因型。
Objective To study the relationship between the different clinical manifestations of cytomegalovirus (HCMV) infection and cytomegalovirus glycoprotein B (gB) genotype in infants. Methods 107 cases of HCMV-infected infants with clinical symptoms and 25 infants without clinical symptoms were enrolled. The gB gene fragment was amplified by nested PCR and the gB gene was genotyped by restriction fragment length polymorphism (RFLP) Some samples were verified by sequencing. Results In 107 infants with clinical symptoms, 53 cases of gB Ⅰ, 20 cases of gB Ⅱ, 18 cases of gBⅢ, 7 cases of gB Ⅰ and Ⅱ mixed, 5 cases of gB Ⅰ and Ⅲ mixed, gB Ⅱ and Ⅲ Mixed 4 cases, no gB Ⅳ type found. Among 53 infants with liver dysfunction, 36 cases were gB Ⅰ, 5 cases gB Ⅱ, 7 cases gB Ⅲ, 3 cases gB Ⅰ and Ⅱ mixed and 2 cases gB Ⅰ and Ⅲ mixed. Accounted for the majority (P <0.05). In 25 infants without clinical symptoms, 10 cases of gB type Ⅰ, 6 cases of gB Ⅱ type, 8 cases of gB Ⅲ type and 1 case of mixed type of gB Ⅰ and Ⅱ were detected in 25 infants without clinical symptoms. Totally 24 samples of 132 infants were sequenced. The results showed that the similarities were 97.06% -99.64% with those of the corresponding typical strains. Conclusion The RFLP genotyping of HCMV gB gene is clear and reliable. In HCMV-infected infants with impaired hepatic function, gB type I was the predominant genotype.