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目的:采用高效液相色谱法测定小儿白贝止咳糖浆中原阿片碱的含量。方法:色谱柱:Bonchrom C_(18)(250 mm×4.6mm,5μm);流动相:乙腈-0.4%磷酸(20:80);检测波长:289 nm。结果:原阿片碱进样量在0.087～0.870μg范围内,呈良好的线性关系(r=0.999 9)。平均加样回收率为98.8%,RSD=0.84%(n=6)。结论:方法易于操作,结果准确,重复性良好,可用于小儿白贝止咳糖浆的质量控制。
Objective: To determine the content of protopine in pediatric cough syrup by high performance liquid chromatography. Method: Column: Bonchrom C 18 (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-0.4% phosphoric acid (20:80); detection wavelength: 289 nm. Results: The original opium alkali injection volume in the range of 0.087 ~ 0.870 μg, showed a good linear relationship (r = 0.999 9). The average recovery was 98.8%, RSD = 0.84% (n = 6). Conclusion: The method is easy to operate with accurate results and good reproducibility. It can be used for the quality control of cough syrup in children.