目的研究68株临床分离表皮葡萄球菌大环内酯耐药性与生物膜形成能力、icaA基因型之间的关系,初步预测采用大环内酯抗菌药物预防表皮葡萄球菌生物膜感染的有效性。方法以琼脂平板稀释法测定菌株的最小抑菌浓度,采用微孔测定法考察临床分离表皮葡萄球菌生物膜形成能力,通过PCR法测定icaA基因型,探讨三者之间的关系。结果临床分离表皮葡萄球菌对大环内酯耐药程度较高(88.2%),且耐药菌生物膜形成能力较敏感菌显著增强(P<0.05),耐药菌与敏感菌的icaA阳性比率没有统计学差异(P>0.05)。结论红霉素、阿奇霉素、克拉霉素等常用的大环内酯类药物可能难以有效地预防表皮葡萄球菌生物膜感染。 Objective To study the relationship between drug resistance of 68 clinical isolates of Staphylococcus epidermidis and biofilm formation ability and icaA genotypes and to predict the effectiveness of macrolide antibiotics in preventing S. epidermidis biofilm infection. Methods The minimum inhibitory concentration (MIC) of the strains was determined by agar plate dilution method. The biofilm formation ability of Staphylococcus epidermidis was investigated by micropore assay. The genotypes of icaA were determined by PCR, and the relationship among the three was explored. Results The clinical isolates of Staphylococcus epidermidis were more resistant to macrolides (88.2%), and the biofilm-forming ability of resistant strains was significantly higher than that of susceptible ones (P <0.05). The positive ratio of icaA to susceptible and susceptible strains No statistical difference (P> 0.05). Conclusion The commonly used macrolides such as erythromycin, azithromycin and clarithromycin may be difficult to effectively prevent S. epidermidis biofilm infection.