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目的检测益生菌(含嗜酸乳杆菌和乳双歧杆菌)对小鼠特异性及非特异性免疫功能的影响。方法 SPF昆明种小鼠经口连续分别给予0.25、0.50、1.50g/kgbw的益生菌30d后,进行小鼠免疫器官重量的测定、脾淋巴细胞转化试验、脾细胞抗体生成试验、肠粘膜sIgA含量测定、腹腔巨噬细胞吞噬荧光微球试验和NK细胞活性测定。结果脾淋巴细胞转化试验中,高剂量组光密度差值高于对照组,差异有显著性意义(P<0.05);高剂量组肠粘膜sIgA含量显著高于对照组(P<0.05);中、高剂量组的NK细胞活性均高于对照组,且差异有显著性意义(P<0.01)。结论摄入一定剂量的益生菌,可增强小鼠特异性及非特异性免疫功能。
Objective To detect the effects of probiotics (including Lactobacillus acidophilus and Bifidobacterium lactis) on the specific and non-specific immune function in mice. Methods SPF Kunming mice were orally administered 0.25, 0.50 and 1.50 g / kg bw of probiotics continuously for 30 days, respectively. The weight of immune organs, splenic lymphocyte transformation test, antibody production test of spleen cells, sIgA content of intestinal mucosa Determination, peritoneal macrophage phagocytosis fluorescent microspheres assay and determination of NK cell activity. Results The difference of optical density in high-dose group was higher than that in control group (P <0.05). The content of sIgA in high-dose group was significantly higher than that in control group (P <0.05) , NK cell activity of high dose group were higher than the control group, and the difference was significant (P <0.01). Conclusion Ingesting a certain dose of probiotics can enhance the specificity and non-specific immune function in mice.