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通过制备拆分剂氯化(8S,9R)-(-)-N-苄基辛可尼定,拆分了外消旋联萘酚得到其两种对映异构体,以手性联萘酚为原料分别合成了配体(R)-L和(S)-L及其相应的Eu(Ⅲ)配合物(R or S)-L-Eu(NO3)3,并测定了旋光度。分别研究了配合物(R or S)-L-Eu(NO3)3与ct-DNA的作用方式及键合常数,发现配合物与小牛胸腺DNA(ct-DNA)存在插入作用,且(R)-L-Eu3+配合物与ct-DNA的键合能力较强。由于ct-DNA对(R)-L-Eu3+配合物荧光的增敏作用,选择(R)-L-Eu3+配合物作为ct-DNA的手性荧光探针,在最佳实验条件下,其线性范围为0.11×10-5～0.55×10-5 mol·L-1,0.55×10-5～7.8×10-5 mol·L-1,检出限为5.85×10-7 mol·L-1。
The enantiomers of racemic binaphthol were resolved by preparation of a resolving agent, (8S, 9R) - (-) - N-benzylcinchonidine. Chiral binaphthyl The ligands (R) -L and (S) -L and their corresponding Eu (Ⅲ) complexes (R or S) -L-Eu (NO3) 3 were synthesized with phenol as the starting material and their optical rotations were measured. The mode of action and the bonding constant of the complex (R or S) -L-Eu (NO3) 3 and ct-DNA were studied respectively. The insertion of the complex with calf thymus DNA (ct- ) -L-Eu3 + complexes and ct-DNA binding ability. Due to the sensitization effect of ct-DNA on the fluorescence of (R) -L-Eu3 + complexes, (R) -L-Eu3 + complexes were selected as chiral fluorescent probes for ct-DNA. Under the optimum conditions, The detection limit was 5.85 × 10-7 mol·L-1 in the range of 0.11 × 10-5 ~ 0.55 × 10-5 mol·L-1, 0.55 × 10-5 ~ 7.8 × 10-5 mol·L-1 .