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用细胞培养术，以ＮａＣＮ加缺糖引起ＰＣ１２细胞损伤作为细胞缺血性损伤的模型，观察蝙蝠葛苏林碱（ＤＳ）及其３个光学异构体对ＰＣ１２细胞缺血性损伤的保护作用；以Ｆｕｒａ２／ＡＭ为Ｃａ２＋的荧光探针，用ＡＲＣＭＭＩＣ阳离子测定系统观察ＤＳ及异构体对ＮａＣＮ引起的Ｃａ２＋内流的抑制作用。结果表明ＤＳ及３个异构体在１０－８～１０－５ｍｏｌ·Ｌ－１的浓度范围内对ＰＣ１２细胞损伤有较明显的保护作用，其机制之一与抑制ＮａＣＮ加缺糖诱发的细胞内游离Ｃａ２＋异常升高有关。
PC12 cell injury was induced by NaCN and glucose deprivation as a model of cell ischemic injury by cell culture. The protective effect of daunorubicin (DS) and its three optical isomers on the ischemic injury of PC12 cells was observed. Fura 2 / AM is a Ca2 + fluorescent probe, AR CM MIC Cation determination system to observe the inhibitory effect of DS and isomers on Ca2 + influx induced by NaCN. The results showed that the DS and three isomers in the concentration range of 10-8 ~ 10-5mol · L-1 on PC12 cell injury have a more significant protective effect, one of the mechanisms and inhibition of NaCN plus glucose-induced intracellular Free Ca2 + abnormal increase.