论文部分内容阅读
目的:探讨木黄酮对人喉癌细胞Hep-2的诱导凋亡及抑制作用。方法:采用CCK-8法测定木黄酮抑制细胞系Hep-2增殖的IC50值;应用流式细胞术检测木黄酮对喉癌细胞所引起的周期分布变化与凋亡率;倒置相差显微镜下观察木黄酮干预人喉癌细胞Hep-2前后的细胞形态学变化。结果:木黄酮抑制Hep-2细胞增殖的IC50值为23.64μg/ml;采用浓度为12μg/ml和24μg/ml的木黄酮分别作用于喉癌细胞24h的凋亡率为22.4%±1.65%和30.64%±2.95%,与对照组比较差异均有统计学意义(P<0.05);采用浓度为12μg/ml和24μg/ml的木黄酮分别作用于喉癌细胞48h的凋亡率为30.55%±0.72%和48.69%±1.06%,与对照组比较差异均有统计学意义(P<0.05)。同一浓度的木黄酮作用于喉癌细胞24、48h的凋亡率差异有统计学意义(P<0.05)。结论:木黄酮能够明显抑制人喉癌细胞Hep-2细胞增殖,并引起Hep-2细胞的凋亡,凋亡率随时间的延长和剂量的增加而增高。
Objective: To investigate the induction of apoptosis and the inhibitory effect of genistein on human laryngeal carcinoma cell line Hep-2. Methods: The IC50 values of proliferation and proliferation of Hep-2 cells were determined by CCK-8. The changes of cell cycle distribution and apoptosis induced by genistein on laryngocarcinoma cells were detected by flow cytometry. Under inverted phase contrast microscope Effect of Flavone on Cell Morphology Before and After Human Laryngeal Cancer Cell Hep-2. Results: The IC50 value of genistein on the proliferation of Hep-2 cells was 23.64μg / ml. The apoptotic rate of Hep-2 cells treated with 12μg / ml and 24μg / ml of genistein for 24 hours was 22.4% ± 1.65% 30.64% ± 2.95%, respectively, which were significantly different from those of the control group (P <0.05). The apoptosis rate of the laryngeal carcinoma cells treated with 12μg / ml and 24μg / ml of genistein for 48h was 30.55% ± 0.72% and 48.69% ± 1.06% respectively, which were significantly different from the control group (P <0.05). The same concentration of genistein on the apoptosis rate of laryngeal carcinoma cells for 24,48h had statistical significance (P <0.05). Conclusion: Genistein can significantly inhibit Hep-2 cell proliferation and induce apoptosis in Hep-2 cells. The apoptotic rate increases with time and dose.