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取13—17天胎鼠脑皮质,以不同的制作条件移植给成年大鼠。鲜植组,将鲜取的供体立即植入。冻植组,将供体冻存(-196℃)5～7天后再植入。存活动物一年后活杀,作光镜、电镜和电子探针观察分析。鲜植组植入物内见成熟神经细胞、其形态与受体细胞有别,纤维向受体脑内延伸;超微观察见神经细胞胞浆丰富,亚膜结构正常。冻植组则见供受体间大多有界限,有的似有一层软脑膜样结构,短纤维为主,有胶质增生;超微观察见神经细胞少,突触短小,有胶质结节。表明冻植组移植结果比较差。胎供体超低温保存尚需进一步深入研究。
Fetal cortex of fetus for 13-17 days was taken and transplanted to adult rats under different production conditions. Fresh plant group, fresh donor immediately implanted. Frozen group, the donor cryopreservation (-196 ℃) 5 to 7 days before implantation. Survival animals killed after one year, for light, electron microscopy and electron probe observation and analysis. In the fresh implants, mature neurons were seen in the implants. The morphology was different from that of the recipient cells, and the fibers extended to the brain of the recipient. The ultrastructure showed that the neurons were abundant in cytoplasm and had normal sub-membrane structure. Frozen group is seen for most of the boundaries between receptors, and some seem to have a layer of pia mater-like structure, mainly short staple, with glial hyperplasia; ultramicro observed less neurons, synapses short, glial nodules . The results showed that the frozen-thawed group had poor results. Cryopreservation of fetal donor needs further study.