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应用组织培养技术保存茶树种质资源，是当今人们正在探索的一种有效、安全的新途径。茶树茎切段试管苗保存技术的研究结果表明，诱导茎切段培养基以ＭＳ或改良ＥＲ基本培养基附加ＢＡ０．０４ｍｇ／Ｌ－４．００ｍｇ／Ｌ＋ＮＡＡ０．０４ｍｇ／Ｌ－０．５０ｍｇ／Ｌ为宜。茎切段的芽诱导率在品种间或同一品种不同季节间存在着明显的差异。茎外植体采样以春梢为好。弱光照、较低温和适当添加化学抑制剂（如甘露醇、ＰＰ＿３３３、ＡＢＡ等）有利于延缓培养物生长、增加培养物保存期。
The application of tissue culture technology to preserve tea tree germplasm resources is an effective and safe new approach that people are exploring today. The results of in vitro culture of tea tree stem cuttings showed that BA0.04mg / L-4.00mg / L + NAA0.04mg / L-0.50mg / L was added as MS medium or modified ER basic medium should. The shoot induction rate of stalks was significantly different among varieties or in different seasons of the same variety. Stem explants sampling spring shoots as well. Weak light, lower temperature and proper addition of chemical inhibitors (such as mannitol, PP_333, ABA, etc.) is conducive to slow the growth of the culture, increase the shelf life of the culture.