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目的研究骨桥蛋白(OPN)介导类风湿关节炎(RA)发病和促进炎症因子产生的作用。方法采用实时定量聚合酶链反应(PCR)的方法检测OPN在RA患者外周血、关节液和关节滑膜组织和在T淋巴细胞亚群中的表达;从蛋白水平分析RA患者关节液中促炎症细胞因子和抗炎症细胞因子的分泌格局,分析OPN与细胞因子的相关性。结果RA患者关节滑液和关节滑膜组织的OPN表达远远高于外周血单个核细胞的OPN基因表达,CD4+T细胞OPN基因表达均高于CD8+T细胞;RA患者关节液中OPN的表达显著高于血清,白细胞介素(IL)-10,干扰素(IFN)-γ和肿瘤坏死因子(TNF)-α水平比自身血清及对照组血清增高,IL-18和IL-12水平比正常对照组明显升高。结论OPN可能通过调节病灶部位的细胞因子的分泌来调控RA病理过程中的炎症反应。
Objective To investigate the role of osteopontin (OPN) in the pathogenesis of rheumatoid arthritis (RA) and the promotion of inflammatory cytokines. Methods The expression of OPN in peripheral blood, synovial fluid, synovial tissue and T lymphocyte subsets of RA patients were detected by real-time polymerase chain reaction (PCR). The levels of pro-inflammatory Cytokines and anti-inflammatory cytokines secretion patterns, analysis of OPN and cytokine correlation. Results The expression of OPN in synovial and synovial tissues of RA patients was much higher than that of peripheral blood mononuclear cells. The expression of OPN gene in CD4 + T cells was higher than that in CD8 + T cells. The expression of OPN The levels of IL-18 and IL-12 were significantly higher than those of sera and control sera The normal control group was significantly higher. Conclusion OPN may regulate the inflammatory reaction in the pathological process of RA by regulating the secretion of cytokines in the lesion site.