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目的了解吉林省2012年手足口病(Hand,Foot and Mouth Disease;HFMD)来源的柯萨奇病毒A组16型(Coxsackie Virus Group A Type 16,CVA16)基因亲缘关系及氨基酸位点的变异情况。方法收集吉林省2012年73例HFMD临床标本,病毒分离后对其中22株的VP1编码区部分核苷酸进行序列测定和分析。结果 22株CVA16中,12株为B1a基因亚型,10株为B1b基因亚型,12株B1a基因亚型氨基酸同源性为95.0%~97.8%,10株B1b基因亚型氨基酸同源性为94.3%~97.0%;有8株在第23位氨基酸位点发生了亮氨酸(Leucine,L)→蛋氨酸(Methionine,M)的变异,其余14株在该氨基酸位点均为L。结论 CVA16为吉林省HFMD的主要病原体之一,毒株间的同源性较高,均属于B基因群,在系统进化树上分别属于两个进化分支。
Objective To understand the genetic relationship and amino acid variation of Coxsackie Virus Group A Type 16 (CVA16) gene from Hand, Foot and Mouth Disease (HFMD) in Jilin Province in 2012. Methods Seventy-three HFMD clinical specimens were collected from Jilin province in 2012. The nucleotide sequences of some of the 22 VP1 coding regions were sequenced and analyzed after virus isolation. Results Among 22 CVA16 strains, 12 were B1a subtype and 10 were B1b subtype. The homology of 12 B1a subtypes was 95.0% ~ 97.8%. The 10 subunits of B1 B1 subtype were 94.3% -97.0%. Eight of them showed the mutation of Leucine, L and Methionine at the 23th amino acid position, and the other 14 strains were L at the amino acid position. Conclusions CVA16 is one of the major pathogens of HFMD in Jilin Province. The homology between strains is high and belongs to the B gene group, which belong to two evolutionary branches in phylogenetic tree respectively.