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维胺酸(N-4-(hydroxycarbophenyl)-retinamide;RⅡ)在30～50mg/kg的剂量下对大鼠移植性软骨肉瘤有明显治疗作用。 低浓度的RⅡ对HL-60细胞有较弱的分化诱导活性,同时加入S86019可使其活性增强。流式细胞光度术分析表明,RⅡ可使HL-60细胞产生G_1期阻断,与S86019联合应用有良好协同阻断效应,使90％细胞积聚在G_1期,Northern blot分析表明,用RⅡ和S86019联合处理HL-60细胞12h后,c-myc表达明显受到抑制,与DNA合成密切相关的胸腺嘧啶核苷酸合成酶mRNA表达也受到抑制。
N-4- (hydroxycarbophenyl) -retinamide (RⅡ) has a significant therapeutic effect on rat transplanted chondrosarcoma at a dose of 30-50 mg / kg. Low concentrations of RII had weaker differentiation-inducing activity on HL-60 cells, and the addition of S86019 enhanced its activity. Flow cytometry analysis showed that RⅡ could block G_1 phase in HL-60 cells and good synergistic blocking effect in combination with S86019, resulting in 90% of cells accumulating in G_1 phase. Northern blot analysis showed that with RⅡ and S86019 The combined treatment of HL-60 cells after 12h, c-myc expression was significantly inhibited, and DNA synthesis is closely related to thymidine synthase mRNA expression was also inhibited.