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利用基于苯乙酸的一步成苗法，获得了来源于多个品种转基因水稻当代植株的花培苗上百株。对其中两个含ｂａｒ基因插入的转基因粳稻当代植株京引１１９－Ｂ３和京引１１９－Ｂ４的花培植株及其后代进行了详细的研究分析。ｂａｒ基因在所获得的单倍体和二倍体花培植株中均可正常表达出对除草剂Ｂａｓｔａ的抗性。ＰＣＲ、Ｓｏｕｔｈｅｒｎ分析进一步证明导入的ｂａｒ基因在绝大多数花培植株中没有发生变化。即使对粳稻京引１１９－Ｂ４这一有多位点插入的转基因植株，花药培养也可达到使外源基因一次纯合、后代不再分离的目的。结果表明对转基因植株进行花药培养可用于快速纯合外源基因以获得纯系。并就这一技术体系的技术要点进行了讨论。
Using the phenylacetic acid-based one-step seedling method, we obtained over one hundred flower seedlings of contemporary plants from several varieties of transgenic rice. In this study, two cultivars and their progenies of transgenic rice Jingyin 119-B3 and Jingyin 119-B4, which are two transgenic plants with bar gene inserted, were studied in detail. The bar gene normally expressed resistance to the herbicide Basta in both the obtained haploid and diploid flower cultivars. PCR and Southern analysis further confirmed that the introduced bar gene did not change in most of the flower cultivars. Even for the transgenic rice Jing-Yin 119-B4, which has multiple loci, the anther culture can also achieve the purpose of homozygous exogenous gene and no further separation of the offspring. The results show that anther culture of transgenic plants can be used to rapidly homozygously express foreign genes to obtain pure lines. And discussed the technical points of this technical system.