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目的 观察妊娠母体接触可卡因引起的胎儿神经系统发育异常 ,研究宫内暴露可卡因引起的胎儿发育迟缓是否与母体孕期营养不良有关以及胎儿神经系统发育异常与神经递质的变化之间的关系。方法 小鼠妊娠d 8(E8)到d 17(E17) ,每日 2次颈背部皮下注射盐酸可卡因 2 0mg·kg-1·d-1,同时建立饮食对照组和盐水对照组 ,每日 2次注射生理盐水 2 0ml·kg-1·d-1,在此期间记录各组母鼠、胎鼠生理指标。E17取材 ,用HPLC法检测给药组血中可卡因浓度及各组母鼠、胎鼠纹状体多巴胺 (dopamine ,DA)、5 羟色胺 (sero tonin ,5 HT)的浓度。结果 可卡因给药组 (COC)与盐水对照组 (SAL)相比 ,母体体重增长量及总摄食量均减少 ,纹状体多巴胺和 5 羟色胺含量增高 (COCn =16 ,SALn =11,P<0 0 1) ;胎鼠体重、脑重、纹状体重也存在明显差异 (SALn=76 ,COCn =92 ,P <0 0 1)。而可卡因给药组与饮食对照组 (SPF)相比 ,在母鼠摄食量相等、体重增长量无明显差异 (COCn =16 ,SPFn =12 ,P >0 0 1)的情况下 ,胎鼠的各项生理指标均下降 (COCn =92 ,SPFn =6 5 ,P <0 0 1) ,且脑纹状体多巴胺、5 羟色胺水平明显升高 ,分别为 (88± 12 )ng·g-1,(2 4 2± 18)ng·g-1。结论 宫内暴露可卡因可引起子代神经系统发育异常 ,这种发育异常?
Objective To observe the fetal nervous system abnormalities caused by cocaine exposure during pregnancy and to investigate whether the intrauterine fetal cocaine-induced fetal retardation is related to maternal malnutrition during pregnancy and the relationship between fetal nervous system dysfunction and neurotransmitter changes. Methods Mice were d8 (E8) to d17 (E17), and subcutaneous injection of cocaine hydrochloride 200 mg · kg-1 · d-1 twice daily on the back of the neck. The rats in the control group and saline control group Injections of normal saline 20ml · kg-1 · d-1, during which the maternal and fetal rat physiological indicators were recorded. E17 were collected and the concentration of cocaine in the blood was measured by HPLC. The concentration of dopamine (DA) and sero-tonin (5 HT) in the striatum were also measured. Results The body weight gain and total dietary intake of cocaine-administered group (COC) were lower than those of saline control group (COCn = 16, SALn = 11, P <0 There was also a significant difference in body weight, brain weight, and striatum weight (SALn = 76, COCn = 92, P <0.01). On the other hand, compared with the diet control group (SPF), the cocaine administration group showed no significant difference in body weight gain (COCn = 16, SPFn = 12, P> 0.01) (COCn = 92, SPFn = 65, P <0.01). The level of dopamine and serotonin in striatum increased significantly (88 ± 12 ng · g-1, (2 4 2 ± 18) ng · g-1. Conclusions Intrauterine exposure of cocaine can cause abnormalities in the offspring of nervous system development.