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刺梨(Rosa roxburgkii)在贵州分布面广,资源丰富,鲜果的Vc含量为841.58~3541.13mg/100g。近年来在野生刺梨的驯化过程中,发现刺梨白粉病较严重。为了提高果品质量,我们自1983年开始,对刺梨白粉病进行了调查研究。一、刺梨白粉病的初侵染菌源材料与方法:入冬以后,刺梨普遍落叶,但幼苗近地面的叶芽仍不断萌发新叶。为了查明刺梨白粉病菌能否在田间幼苗上度过寒冬,我们分别于1983、1984年冬季,在本院刺梨苗圃基地,定点、定期凋查嫩叶发病率。并于1984年12月上旬,自田间采集新鲜病叶,在大培养皿内用50ppm苯骈咪唑浸湿脱脂棉,将病叶放上面,加皿盖,置于冰箱内,零下1~5℃恒温保绿培养20天,然后取分生孢子用染色法检查孢子成活率;另取分生孢子置于凹玻片的水
Rosa roxburgkii is widely distributed in Guizhou and is rich in resources. The Vc content of fresh fruit is 841.58 ~ 3541.13mg / 100g. In recent years, during the domestication of wild prickly pear, prickly pear powdery mildew was found more serious. In order to improve the quality of fruit, we have investigated the powdery mildew of thorns since 1983. First, pricker powdery mildew primary infection Source materials and methods: After winter, prickly pear generally deciduous, but seedlings near the surface of the leaf bud is still germination of new leaves. In order to find out whether Cili powdery mildew can survive winter in field seedlings, we respectively in 1983, the winter of 1984, in our hospital Creeper nursery base, fixed-point, regularly investigate the incidence of young leaves. And in early December 1984, collected from the field of fresh diseased leaves, in a large Petri dish with 50ppm benzimidazole absorbent cotton, the sick leaves on the top, add the dish cover, placed in the refrigerator, minus 1 ~ 5 ℃ constant temperature Green Paul cultured for 20 days, and then take conidia staining method to check the survival rate of spores; another take conidia placed in a concave glass of water