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目的 :探讨急性心肌梗死患者 (AMI)溶栓前后体内一氧化氮 (NO)、一氧化氮合酶 (NOS)及丙二醛 (MDA)含量变化的临床意义。方法 :采用比色法测定 16例AMI溶栓前后血清NO (硝酸还原酶法 )及NOS含量 ,采用硫代巴比妥酸比色法测定红细胞MDA含量。结果 :AMI溶栓前NO及NOS分别为 37 8± 6 9μmol/L和 19 1± 9 2u/ml,低于对照组 76 9±2 0 3μmol/L和 2 8 3± 11 1u/ml,差异均非常显著 ( P <0 0 1) ;溶栓再通后NO及NOS分别为45 5± 11 3μmol/L和 2 6 6± 10 4u/ml,与溶栓前比较 ,差异均显著 ( P <0 0 5 ) ,但NO仍显著低于对照组 (P <0 0 1) ,NOS与对照组比较已无差异 (P >0 0 5 ) ;溶栓前MDA为 0 334±0 0 98nmol/ml,高于对照组 0 2 2 8± 0 0 31nmol/ml,溶栓后MDA进一步升高。结论 :NO、NOS及MDA参与了AMI的发生和发展过程 ,测定AMI患者的NO、NOS及MDA的变化可能对病情的判断具有重要意义
Objective: To investigate the clinical significance of the changes of nitric oxide (NO), nitric oxide synthase (NOS) and malondialdehyde (MDA) in patients with acute myocardial infarction (AMI) before and after thrombolysis. Methods: Serum NO (nitrate reductase) and nitric oxide synthase (NOS) contents were measured by colorimetric assay before and after thrombolytic therapy in 16 cases. The content of MDA in erythrocytes was measured by thiobarbituric acid colorimetry. Results: Before AMI, NO and NOS were 37 8 ± 6 9μmol / L and 19 1 ± 92uu / ml, respectively, which were lower than 76 9 ± 20 3μmol / L and 2 8 3 ± 11 1u / ml in control group (P <0.01). NO and NOS after reperfusion were 45 5 ± 11 3μmol / L and 266 ± 104u / ml, respectively, which were significantly different from those before thrombolysis (P < 0.05), but NO was still significantly lower than that of the control group (P <0.01). NOS had no significant difference compared with the control group (P> 0.05); MDA before the thrombolysis was 0 334 ± 0 98 nmol / ml , Which was higher than that of the control group (0 2 2 8 ± 0 0 31 nmol / ml). After thrombolysis, MDA increased further. Conclusions: NO, NOS and MDA are involved in the occurrence and development of AMI. It is of great significance to determine the changes of NO, NOS and MDA in patients with AMI