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目的:观察灭蚊真菌大链壶菌感染蚊幼虫后两者的组织形态学变化以了解该真菌致死蚊幼虫的机理。方法:用真菌实验室感染蚊幼虫后取不同感染程度和时间的蚊幼虫作常规组织切片,H.E.染色,然后以正常蚊幼虫为对照,用比较组织学方法观察受染蚊幼虫和菌体的组织学变化。结果:在较早的Ⅰ度感染蚊幼虫,可见蚊头,胸部组织间隙有较细的、不分隔的大链壶菌营养型菌丝体,后者进而在血体腔内大量繁殖,并侵入肌肉、脂肪体、胃、肠等组织和细胞内,被入侵处的组织发生破坏和消失;Ⅱ度感染的蚊幼虫体内,可见蚊组织被严重破坏甚至消失,菌丝体发育为生殖型,即菌丝出现分隔,变粗变短或形成圆形、卵圆形或串珠状的孢子体。感染时间较长时蚊体内正常结构大部分消失,而此时菌丝体和孢子体内的原生质和核也已消失,只剩下中空的细胞壁管道。结论:认为大链壶菌对蚊幼虫组织的直接入侵和破坏是该菌致蚊幼虫死亡的主要原因之一。
Objective: To observe the morphological changes of mosquito larvae infected with mosquitoes, and to understand the mechanism of mosquito larvae killing by mosquitoes. Methods: Mosquito larvae were inoculated with fungi in laboratory, and the mosquito larvae with different infection degree and time were taken as normal tissue sections. E. Staining, and then normal mosquito larvae as a control, by comparative histological observation of mosquito larvae and bacterial histological changes. Results: In earlier stage I mosquito larvae, mosquito head and chest tissue were observed with thin, non-segmented kallikra-vegetative mycelium, which in turn multiplied in the blood cavity and invaded the muscle , Fat body, stomach, intestine and other tissues and cells were destroyed and disappeared by the invasion of the organization; Ⅱ degree infected mosquito larvae, we can see the mosquito was severely damaged or even disappear, mycelium developed into reproductive type, that bacteria Silks appear separated, becoming shorter or thicker to form round, oval or beaded sporozoites. Most of the normal mosquitoes disappeared when the infection time was longer, but at this time, the protoplasm and nucleus in the mycelium and spores disappeared, leaving only the hollow cell wall. Conclusion: It is considered that the direct invasion and destruction of Lagerstrum against mosquito larvae are one of the main causes of the mosquito larvae ’s death.