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目的应用胶体金法快速法(TPCG)检测血清梅毒抗体,并与梅毒螺旋体明胶颗粒凝集试验(TPPA)进行比较,对胶体金快速法的应用性进行评价。方法对29 640例(A组)需要检测梅毒的患者血清,用TPPA法检测,对结果阳性的用TPCG法复查。对4892例(B组)需要应用快速法检测梅毒的患者血清,用TPCG法检测,对结果阳性者用TPPA法复查。对8份梅毒抗体阳性血清用阴性血清进行倍比稀释,对稀释后的同一份血清分别用TPPA法和TPCG法进行定性检测,以比较TPCG法的灵敏度和可检测范围。结果 A组TPPA法检出616例阳性,用TPCG复查612例阳,阳性符合率为99.35%;阳性漏检率为0.01%;B组TPCG法共检测出91例阳性,用TPPA法复查80例阳性,符合率为87.9%。TPCG法能检测到TPPA法下限的1/2~1/4,可从下限开始检测出下限的1024倍或更高。结论 TPCG法敏感度高,漏检率低,试剂易于保存,独立包装,操作简单,结果易于判断,能满足基层医院筛查的需要,对于阳性结果,应结合症状,必要时可以到上级医院复查。
OBJECTIVE: To evaluate the applicability of colloidal gold rapid method by using the method of colloidal gold method (TPCG) to detect the serum syphilis antibody and compare with the Treponema pallidum gelatin particle agglutination test (TPPA). Methods Totally 29 640 cases (group A) of patients with syphilis were tested for sera by TPPA assay and positive for TPCG assay. 4892 cases (group B) need to apply rapid test for syphilis in patients with serum, using TPCG test, the results were positive with TPPA review. Eight copies of syphilis antibody-positive serum were diluted with negative serum, and the same serum was diluted with TPPA method and TPCG method respectively to compare the sensitivity and detectable range of TPCG method. Results A total of 616 positive cases were detected by TPPA in group A, 612 cases were positive by TPCG test, and the positive coincidence rate was 99.35%. The negative detection rate was 0.01%. In group B, 91 cases were positive by TPCG assay and 80 cases by TPPA test Positive, in line with the rate of 87.9%. The TPCG method can detect ½ to ¼ of the lower limit of the TPPA method and can detect 1024 times or more of the lower limit from the lower limit. Conclusion TPCG method has the advantages of high sensitivity, low missed detection rate, easy storage of reagents, independent packaging, simple operation, easy determination of results and the ability to meet the needs of primary hospital screening. For positive results, symptoms should be combined, and if necessary, .