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作者从黄岩等地采集到的金针菇软腐病病菇上,分离病原菌时发现有线虫存在,进一步分离得到线虫活体。一、线虫形态特征:雌、雄成虫虫体线状,雌虫长0.61～0.98毫米。宽0.027～0.039毫米;雄虫长0.69～0.87毫米,宽0.032～0.037毫米。虫体白色,头部口器中无吻针,尾细长,为杆形目线虫。二、线虫对金针菇菌丝及菇体致病力:分离获得的线虫活体经体表消毒后接入 PDA 斜面金针菇菌落中,在18～20℃下培养,先可见线虫取食菌丝而使菌落逐渐稀淡,后可发现线虫危害斜面上金针菇菇体后留下的
The authors collected from Huangyan and other soft-rotted mushroom Flammulina, isolated from pathogens found nematodes exist, further isolation of nematode living. First, the nematode morphological characteristics: male and female adult worms linear, female 0.61 ~ 0.98 mm long. Width 0.027 ~ 0.039 mm; male length 0.69 ~ 0.87 mm, width 0.032 ~ 0.037 mm. Insect white, no mouth kiss in the mouth, tail slender, for the rod-shaped nematode. Second, nematodes on Flammulina velutipes and mushroom body pathogenicity: Isolated nematode living body after disinfection by surface access to PDA bevel flammulina colonies, cultured at 18 ~ 20 ℃, first visible nematodes feeding mycelium and colonies Gradually dilute, after the nematode can be found on the bevel mushrooms harm the body left behind