目的:建立肠炎清合剂中君药黄连的鉴别及盐酸小檗碱、盐酸巴马汀含量测定方法。方法:采用薄层色谱法对合剂中黄连进行定性鉴别;采用HPLC进行含量测定。结果:薄层色谱均能明显的检出黄连的特征有效成分盐酸小檗碱和盐酸巴马汀;HPLC法中,盐酸小檗碱在1.34～42.8μg.mL-1内线性关系良好(r=0.9999),平均回收率99.3%,RSD=0.92%(n=9);盐酸巴马汀在1.24～39.8μg.mL-1内线性关系良好(r=0.9999),平均回收率98.6%,RSD=0.82%(n=9)。结论:本法简便、快速、灵敏、准确,重现性好,可以作为该合剂的质量控制方法。 Objective: To establish the identification of Rhizoma Coptidis (Rhizoma Coptidis) in Changyinqing Mixture and the determination of berberine hydrochloride and palmatine hydrochloride. Methods: TLC was used for the qualitative identification of Coptis chinensis Franch. HPLC was used to determine the content of Coptidis Rhizoma. Results: By TLC, berberine hydrochloride and palmatine hydrochloride could be clearly detected. Berberine hydrochloride showed a good linearity within 1.34 ~ 42.8μg.mL-1 (r = 0.9999). The average recovery was 99.3% with RSD = 0.92% (n = 9). The linearity of palmatine hydrochloride was 1.24 ~ 39.8μg.mL-1 with the average recovery of 98.6% 0.82% (n = 9). Conclusion: The method is simple, rapid, sensitive, accurate and reproducible. It can be used as the quality control method of the mixture.