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目的 观察兔睾丸扭转复位后睾丸细胞凋亡情况及药物减轻睾丸凋亡的效果。方法 选用青春期雄性日本大耳白兔 2 0只 ,体重 90 0～ 110 0g ,月龄 2～ 3个月 ,随机分为 4组 ,每组 5只。手术制作幼兔睾丸扭转模型 (72 0° ,2h) ,对部分扭转组应用抗氧化剂 (抗坏血酸 )或钙离子通道拮抗剂 (维拉帕米 )。 2d后取出睾丸 ,TUNEL法观查各组动物睾丸组织细胞凋亡情况 ,HE染色检查睾丸病理组织学改变。结果 幼兔睾丸扭转复位后 ,精原细胞发生凋亡 ,而睾丸支持细胞及间质细胞未见凋亡现象。单纯扭转组精原细胞平均凋亡指数 (AI) (7.92± 1.2 9)‰ ,较对照组 (2 .6 0± 1.0 1)‰显著升高 ;睾丸扭转前后应用抗坏血酸及维拉帕米组的睾丸细胞平均AI ,分别为 (4.12± 0 .73)‰ ,(4.0 8± 0 .88)‰ ,较单纯扭转组 (7.92± 1.2 9)‰显著下降。HE染色的组织学改变与TUNEL法类似。结论 睾丸扭转损伤后可致使生精细胞凋亡。应用抗氧化剂及钙离子通道拮抗剂可以减轻扭转睾丸的精原细胞凋亡
Objective To observe the apoptosis of testicular cells after testicular torsion and reduce the testicular apoptosis. Methods Twenty adolescent male Japanese white rabbits weighing 90 to 110 g and 2 to 3 months of age were randomly divided into 4 groups (n = 5 each). The model of testicular torsion in rabbits was made by surgery (72 0 °, 2 h). Anti-oxidant (ascorbic acid) or calcium channel antagonist (verapamil) was applied to partial torsion group. After 2 days, the testis was removed. TUNEL method was used to observe the apoptosis of testicular tissue in each group. HE staining was used to examine the histopathological changes of testis. Results After testicular torsion and degeneration, the apoptosis of spermatogonia occurred in testis and no apoptosis occurred in testicular supportive cells and interstitial cells. The average apoptotic index (AI) of spermatogonia in pure torsion group (7.92 ± 1.2 9) ‰ was significantly higher than that in control group (2.60 ± 1.0 1) ‰, and ascorbic acid and verapamil in testis before and after torsion The average AI of testicular cells was (4.12 ± 0.73) ‰, (4.08 ± 0.88) ‰, which was significantly lower than that of pure torsion group (7.92 ± 1.2 9) ‰. Histological changes in HE staining were similar to TUNEL. Conclusion Testicular torsion injury can lead to apoptosis of spermatogenic cells. Application of Antioxidants and Calcium Channel Antagonists Ameliorate Testis-Induced Apoptosis of Spermatogonia