论文部分内容阅读
以榨菜细胞质雄性不育系(CMS)为不育胞质来源,甘蓝型油菜自交系为父本,通过连续回交定向选育方法进行榨菜胞质甘蓝型油菜CMS的转育,获得了原始不育系‘93-12A’,并通过父本载体的转换对其进行改良,获得了一份综合性状较优良的不育系‘嘉油58A’;根据已报道的油菜CMS相关基因orf224、orf138和叶用芥菜CMS相关基因orf220设计特异引物,对榨菜胞质甘蓝型油菜CMS及其保持系的基因组DNA进行PCR扩增,结果显示,仅orf220的特异引物能在原始不育系‘93-12A’和改良不育系‘嘉油58A’中扩增出特异条带,扩增产物序列与orf220具有100%的同源性,从而从分子水平验证‘93-12A’和‘嘉油58A’的不育胞质来源于榨菜。
The cytoplasmic male sterile line (CMS) was used as a source of sterile cytoplasm, and the inbred line of Brassica napus was used as the male parent. Through continuous backcross selection and breeding method, CMS CMS-93 -12A ’, and transformed it by male-parent vector to obtain a good CMS line’ Jiayou 58A ’. According to the reported CMS-related genes orf224, orf138 And leaf mustard CMS-related genes orf220 design specific primers for the Brassica napus CMS and its maintainer line genomic DNA were PCR amplified, the results show that only orf220 specific primers can be in the original line ’93 -12A ’And improved CMS’ Jiayou 58A ’. The amplified products have 100% homology with orf220, so as to validate ’93 -12A’ and ’Jiayou 58A’ from the molecular level Sterile cytoplasm from the mustard.